Invitrogen™ Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, streptavidin

Invitrogen™ Alexa Fluor™ 555 Tyramide SuperBoost™ Kit, streptavidin

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 555 tyramide (555/565 ex/em), detected using a standard Orange/RFP/TRITC filter cube. This kit also features HRP-conjugated streptavidin.

Features

• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Contents

• Blocking buffer (1 X), 22.5 mL
• HRP-conjugated streptavidin (1 X), 22.5 mL
• Alexa Fluor tyramide reagent
• Hydrogen peroxide (stabilized 3% solution), 28.5 mL
• Reaction buffer (20 X), 6 mL
• Reaction stop solution, 8 mg
• Dimethylsulfoxide (DMSO), 200 µL