TPP Techno Plastic Products™ Pierce™ LysN Protease, MS Grade

TPP Techno Plastic Products™ Pierce™ LysN Protease, MS Grade

Thermo Scientific Pierce LysN Protease, MS Grade, is highly purified native Lys-N protease that has been validated for maximum activity and stability in proteomic applications.

Thermo Scientific Pierce LysN is a mass spectrometry (MS)-grade zinc metalloprotease derived from Grifola frondosa. The native Pierce LysN Protease has been highly purified to improve stability, specific activity, and cleavage selectivity. Unlike trypsin, LysN protease cleaves at the amino-terminus of lysine residues. As a result, the peptides generated by LysN are longer than those generated by trypsin and have more prevalent charged amino terminal peptide fragments. Additionally, LysN protease is more promising for epigenetic MS applications than LysC or trypsin because it is capable of cleaving methylated lysines [1]. The thermostability and chemical compatibility with denaturants make LysN protease ideal for digestion of complex protein samples for “shotgun” proteomics.

Features

• Thermostable – provides better digestion at higher temperatures (e.g., 50°C) in less time
• Complementary to tryptic digests – cleaves the opposite side of lysine from trypsin
• High purity – no additional (off-target) protease activity detected
• N-terminal lysine cleavage specificity – at least 90% for a complex protein sample
• Versatile – enzyme is effective at many temperatures and in denaturing conditions
• Stable – provided in a lyophilized format