Invitrogen™ Amino Allyl MessageAmp™ II aRNA Amplification Kit

Invitrogen™ Amino Allyl MessageAmp™ II aRNA Amplification Kit

The Amino Allyl II MessageAmp™ aRNA Amplification Kit includes reagents for aRNA amplification with amimo allyl NTP incorporation. The kit includes sufficient reagents for 20 reactions. Cy™ dyes are not included.

Included in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript™ RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.

Contents

• T7 Oligo(dT) Primer (-20°C): 60 µl 
• ArrayScript™ Reverse Transcriptase (-20°C): 22 µl 
• RNase Inhibitor (-20°C): 22 µl 
• 10X First Strand Buffer (-20°C): 42 µl 
• dNTP Mix (-20°C): 170 µl 
• 10X Second Strand Buffer (-20°C): 210 µl 
• DNA Polymerase (-20°C): 42 µl 
• RNase H (-20°C): 22 µl 
• T7 Enzyme Mix (-20°C): 84 µl 
• 10X Reaction Buffer (-20°C): 84 µl 
• 50 mM UTP Solution (-20°C): 95 µl 
• 50 mM 5-(3-amino allyl)-UTP (-20°C): 64 µl 
• ATP, CTP, GTP Mix (-20°C): 255 µl 
• Second Round Primers (-20°C): 40 µl 
• 1 mg/ml Control RNA (-20°C): 10 µl 
• Nuclease-free Water (any temperature): 1.75 ml 
• Coupling Buffer (-20°C): 400 µl 
• DMSO (-20°C): 440 µl 
• 4M Hydroxylamine (-20°C): 180 µl 
• Wash buffer (4°C or room temperature): 40 ml 
• cDNA Binding Buffer (room temperature): 7 ml 
• aRNA Binding Buffer (room temperature): 20 ml 
• aRNA Filter Cartridges (room temperature): 20 Cartridges
• aRNA Collection Tubes (room temperature): 40 Tubes
• cDNA Filter Cartridges + Tubes (room temperature): 20  Cartridges + Tubes 
• cDNA Elution Tubes (room temperature): 20 Tubes
• Nuclease-free Water (any temperature): 10 ml 
• Labeled aRNA Filter Cartridges + Tubes (room temperature): 20 Cartridges + Tubes 
• Labeled aRNA Elution Tubes (room temperature): 20 Tubes