Invitrogen™ ARES™ Alexa Fluor™ 647 DNA Labeling Kit

Invitrogen™ ARES™ Alexa Fluor™ 647 DNA Labeling Kit

The ARES™ Alexa Fluor® DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor® dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor® 647 dye. The labeled probes can be used for fluorescence in situ hybridization (FISH) and microarray techniques. We offer ARES™ Alexa Fluor® DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5 µg DNA each.
ARES™ Alexa Fluor® Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in our FISH Tag™ DNA and FISH Tag™ RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.

Features

• Dye (Ex/Em): Alexa Fluor® 647 (650/670 nm)
• Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides
• Typically produces one dye per 12–20 bases
• Optimal for FISH and microarrays
  
  

Contents

• 5-(3-aminoallyl)-dUTP (Component A): 50 μL
• Reactive dye (Component B): 10 vials
• Dimethylsulfoxide (DMSO) or dimethylformamide (DMF) (Component C): 200 μL
• Sodium bicarbonate (MW = 84.0) (Component D): 84 mg
• Nuclease-free water (Component E): 10 mL