Invitrogen AMPK alpha-1,2 (Phospho) [pT172] Human ELISA Kit, 96 Tests
On demandInvitrogen AMPK alpha-1,2 (Phospho) [pT172] Human ELISA Kit, 96 Tests
The Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of AMPK alpha-1,2 (Phospho) [pT172] in fresh or frozen human cell lysates. Cross-reactivity has been observed in mouse and rat cells. The assay recognizes both natural and recombinant human AMPK alpha-1,2 (Phospho) [pT172].
Principle of the method
A
monoclonal capture antibody specific for AMPK alpha-1,2 (Phospho)
[pT172] has been coated onto the wells of the 96-well plate. During the
first incubation, standards of known content and unknown samples are
pipetted into the wells and the antigen binds to the immobilized
(capture) antibody. After washing, a rabbit antibody specific for the
target protein is added to the wells and serves as a detection antibody
by binding to the immobilized protein captured during the first
incubation. After washing, a horseradish peroxidase labeled anti-rabbit
IgG is added. This binds to the detection antibody to complete the four
member sandwich. After a third incubation and washing to remove all the
unbound enzyme, a substrate solution (TMB) is added, which is acted upon
by the bound enzyme to produce color. The intensity of this colored
product is directly proportional to the concentration of target protein
present in the original specimen and the optical density can be read on a
standard microplate reader.
Features
- Homogenous (no wash): No
- Interassay CV: 7.6%
- Intraassay CV: 4.8%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- AMPKα [pT172] Standard; contains 0.1% sodium azide. 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide; red dye [1], 25 mL
- Antibody Coated Wells, 96-well plate, 1 plate
- AMPKα [pT172] Detection Antibody; contains 0.1% sodium azide; blue dye[1], 11 mL
- Anti-Rabbit IgG HRP (100X), 0.125 mL
- HRP Diluent. Contains 3.3 mM thymol; yellow dye[1], 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 3
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