Invitrogen Tau (Phospho) [pS199] Human ELISA Kit, 96 Tests
On demandInvitrogen Tau (Phospho) [pS199] Human ELISA Kit, 96 Tests
The Human Tau (Phospho) [pS199] ELISA quantitates Hu Tau (Phospho) [pS199] in human cerebrospinal fluid (CSF), cell lysates, brain homogenates, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Tau (Phospho) [pS199].
Principle of the method
The
Human Tau (Phospho) [pS199] solid-phase sandwich ELISA (enzyme-linked
immunosorbent assay) is designed to measure the amount of the target
bound between a matched antibody pair. A target-specific antibody has
been pre-coated in the wells of the supplied microplate. Samples,
standards, or controls are then added into these wells and bind to the
immobilized (capture) antibody. The sandwich is formed by the addition
of the second (detector) antibody, a substrate solution is added that
reacts with the enzyme-antibody-target complex to produce measurable
signal. The intensity of this signal is directly proportional to the
concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 5.8%
- Intraassay CV: 3.9%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu Tau [pS199] Standard, lyophilized; contains 0.1% sodium azide. 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
- Antibody Coated Plate; 96-well plate, 1 plate
- Hu Tau [pS199] Detection Antibody. Rabbit anti-Tau [pS199]; contains 0.1% sodium azide, 11 mL
- Anti-Rabbit IgG HRP (100X), 0.125 mL
- HRP Diluent; contains 0.1% Kathon™ CG/ICP, 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 3
There are no specifications
There are no report
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