Invitrogen™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit

Invitrogen™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit

The Amplex™ Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer.

The Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate. Because peroxidase- and glucose oxidase-mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay. Because of the ability to couple reactions, this assay has been demonstrated to be useful for the quantification of glucose levels in foods, fermentation media, and bodily fluids.
In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide then reacts with the Amplex™ Red reagent in a 1:1 stoichiometric ratio to generate the red-fluorescent product resorufin.

Features

• Detect concentrations as low as 3 µM d-glucose or 0.05 mU/mL of glucose oxidase
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference