Thermo Scientific™ SILAC Protein Quantitation Kit (Trypsin), RPMI 1640

Thermo Scientific™ SILAC Protein Quantitation Kit (Trypsin), RPMI 1640

The Thermo Scientific SILAC Protein Quantitation Kit (Trypsin), RPMI 1640, is used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

Features

• Efficient—up to 100% label incorporation into proteins of living cells
• Reproducible—minimizes intra-experimental variability caused by differential sample preparation
• Flexible—media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling
• Compatible—label proteins expressed in a wide variety of mammalian cell lines adapted to grow in RPMI 1640 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat, and others
• High-quality supplements—heavy amino acids with >99% isotope purity; dialyzed FBS tested to help ensure that it is sterile, endotoxin-free, and cell culture compatible

Contents

• RPMI 1640 Medium for SILAC, 2 x 500 mL
• 13C6 15N2 L-lysine-2 HCl, 1 x 50 mg
• 13C6 15N4 L-arginine HCl, 1 x 50 mg
• L-lysine-2 HCl, 1 x 50 mg
• L-arginine-HCl, 1 x 50 mg, store at 4°C
• 100 mL FBS, store (at -20°C)