Invitrogen Amyloid beta (Aggregated) Human ELISA Kit, 96 Tests
On demandInvitrogen Amyloid beta (Aggregated) Human ELISA Kit, 96 Tests
The Human Aggregated Amyloid Beta ELISA quantitates Hu aggregated Aβ in human tissue homogenates, ventricular fluid, CSF, tissue culture supernatant, and buffered solution. The assay will exclusively recognize both natural and recombinant Hu aggregated Aβ.
Principle of the method
The Human aggregated Aβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: 7.4%
- Intraassay CV: 7.4%
- Homogenous (no wash): No
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu Aggregated Aβ Standard, lyophilized; contains 0.1% sodium azide, 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide; red dye [1], 60 mL
- Antibody Coated Plate, 96-well plate, 1 plate
- Hu Aggregated Aβ Biotin Conjugate; contains 0.1% sodium azide; blue dye [1], 11 mL
- Streptavidin‑HRP (100X); contains 3.3 mM thymol, 0.125 mL
- HRP Diluent; contains 3.3 mM thymol; yellow dye [1], 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 3
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