Invitrogen ANGPTL4 Human ELISA Kit, 5 x 96 Tests
On demandInvitrogen ANGPTL4 Human ELISA Kit, 5 x 96 Tests
The Human Angiopoietin Like4 (Hu ANGPTL4) ELISA quantitates Hu ANGPTL4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ANGPTL4.
Principle of the method
The Human ANGPTL4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human Angiopoietin like4 Antibody Coated wells, 96-well plate, 1 plate
- Human Angiopoietin like4 Biotin Conjugate, 2 vials
- Human Angiopoietin like4 Standard, recombinant human angiopoietin like4, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (280X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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