Invitrogen™ Annexin V Conjugates for Apoptosis Detection, Alexa Fluor 488
On demandInvitrogen™ Annexin V Conjugates for Apoptosis Detection, Alexa Fluor 488
Annexin V has a high affinity for phosphatidylserine (PS), which becomes
exposed on the outer leaflet of cells undergoing apoptosis. Because of
this affinity, fluorescently labeled annexin V reagents are commonly
used in apoptosis research.
Annexin V conjugates provide quick and reliable detection methods for
studying the externalization of phosphatidylserine, an indicator of
intermediate stages of apoptosis. The difference in fluorescence
intensity between apoptotic and nonapoptotic cells stained with our
fluorescent annexin V conjugates, as measured by flow cytometry, is
typically about 100-fold.
In collaboration with Nexins Research BV, we provide the best and
brightest annexin V conjugates available, including Alexa Fluor 350,
488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as
Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly
fluorescent annexin V conjugates provide quick and reliable detection
methods for studying the externalization of phosphatidylserine, one of
the earliest indicators of apoptosis.
The Annexin V Pacific Blue conjugate is violet excitable, making it
ideal for instruments with a violet laser and for multicolor experiments
that include green- or red-fluorescent dyes. Annexin V staining to detect apoptotic cells can only be done on live
cells and tissue. If samples are to be fixed post-staining, there are
specific conditions required to achieve transient retention of signal.
These include use of an alcohol-free, aldehyde-based fixation method,
use of buffers containing Ca2+ and avoidance of surfactants/detergents.
For your convenience, we also offer a concentrated annexin-binding
buffer that facilitates the binding of annexin V to phosphatidylserine
in apoptosis assays.
Features
- Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals.
- Conjugates for all available lasers.
- Available as stand-alone reagents or easy-to-use kits.
Contents
- 1 vial of annexin V, Alexa Fluor 488 conjugate.
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