Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

ReadyProbes™ Cell Viability Imaging Kit (Blue/Red) is a ready-to-use kit that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue™ Live reagent (Hoechst 33342) and propidium iodide to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set. This kit is appropriate for fluorescence microscopy, fluorescence micro plate readers, and flow cytometry. NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm) Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617 nm)

For Use With (Equipment): Fluorescence Microscope