Invitrogen™ Annexin V Ready Flow Conjugates for Apoptosis Detection, Alexa Fluor 647

Invitrogen™ Annexin V Ready Flow Conjugates for Apoptosis Detection, Alexa Fluor 647

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.
The Annexin V Ready Flow conjugates are provided as ready-to-use, direct-to-sample apoptotic cell stains. Simply add one drop from the dropper bottle directly into 1 x 106 cells in 5 mM Ca2+-containing cell culture medium or annexin binding buffer, incubate, and analyze. The traditional annexin staining protocol can be used as an alternative method by replacing the addition of 5 μL annexin conjugate with the addition of 1 drop from the Ready Flow dropper bottle.
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

Contents

• 2 Dropper bottles.