Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection

Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection

CellROX™ Green Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. The cell-permeant dye is weakly fluorescent while in a reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ∼ 485/520 nm. This reagent can be formaldehyde-fixed and its signal survives detergent treatment, which makes it multiplexable with other compatible dyes and antibodies, CellROX™ Green Reagent is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as our FLoid™, Tali™, and Attune™ instruments

Features

• Compatible with other live cell dyes, antibodies, and in cells expressing RFP
• Provided as a stable, ready-to-use DMSO solution with a simple no-wash protocol compatible with standard workflows in fluorescence microscopy
• Provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips