Invitrogen™ Anza™ 5-Pack Starter Kit

Invitrogen™ Anza™ 5-Pack Starter Kit

The Anza™ Restriction Enzyme Cloning System employs a single buffer and protocol for all Anza restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This eliminates the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Features

• Convenient—all Anza enzymes are 100% functional in the single Anza buffer
• Fast—complete digestion in 15 minutes
• Flexible—overnight digestion without star activity
• Simple—a single digestion protocol for all DNA types
• Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Contents

• 20 µL each of Anza 11 EcoRI, Anza 5 BamHI, Anza 1 NotI, Anza 16 HindIII, Anza 12 XbaI
• 100 µL Anza T4 DNA Ligase Master Mix
• 20 µL Anza Alkaline Phosphatase
• 500 µL Anza Buffer (10X)
• 500 µL Anza Red Buffer (10X)