Invitrogen™ Anza™ Alkaline Phosphatase, 500 Reactions

Invitrogen™ Anza™ Alkaline Phosphatase, 500 Reactions

Invitrogen™ Anza™ Alkaline Phosphatase is intended for use in the removal of 3' and 5' phosphate groups that remain after Anza™ restriction enzyme digestion, such as for the dephosphorylation of vectors prior to insert ligation and removal of 5′ phosphates prior to end-labeling. Since the 5′ phosphate group is required for DNA ligation, treatment of vectors with Anza Alkaline Phosphatase prevents self-ligation and re-circularization, resulting in decreased vector background when cloning.

Features

• Validated for use with all Anza restriction enzymes
• Dephosphorylates in 15 minutes at 37°C
• Heat inactivated after 5 minutes at 80°C
• Fully active in Anza Buffer
• Dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs)

Contnents

• 500 µL Anza Alkaline Phosphatase
• 500 µL Anza Buffer (10X)
• 500 µL Anza Red Buffer (10X)