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Invitrogen™ Anza™ T4 PNK Kit

On demand
Order number : IVGN2304
(Ex-work price)
$ 163

Invitrogen™ Anza™ T4 PNK Kit


The Invitrogen™ Anza™ T4 PNK (polynucleotide kinase) Kit is used to perform 5’-phosphorylation of DNA and oligonucleotides. T4 PNK exhibits 5’ polynucleotide kinase and 3’ phosphatase activity, catalyzing the transfer of the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid.

When preparing DNA for the ligation step in cloning, either the insert DNA or the vector DNA should contain a 5’ phosphate. Often PCR-amplified DNA is treated with T4 PNK to phosphorylate the 5’ end for subsequent cloning ligation.


Features

  • Fast 15-minute incubation at room temperature
  • Phosphorylaton can be performed with DNA or oligonucleotides in water, TE, or 1X Anza buffers

Contents


  • 50 reactions Anza T4 PNK Enzyme
  • 0.5 mL Anza 10X T4 PNK Buffer
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