Invitrogen™ Anza™ T4 PNK Kit
On demand
Order number :
IVGN2304
Invitrogen™ Anza™ T4 PNK Kit
The Invitrogen™ Anza™ T4 PNK (polynucleotide kinase) Kit is used to perform 5’-phosphorylation of DNA and oligonucleotides. T4 PNK exhibits 5’ polynucleotide kinase and 3’ phosphatase activity, catalyzing the transfer of the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid.
When preparing DNA for the ligation step in cloning, either the insert DNA or the vector DNA should contain a 5’ phosphate. Often PCR-amplified DNA is treated with T4 PNK to phosphorylate the 5’ end for subsequent cloning ligation.
Features
- Fast 15-minute incubation at room temperature
- Phosphorylaton can be performed with DNA or oligonucleotides in water, TE, or 1X Anza buffers
Contents
- 50 reactions Anza T4 PNK Enzyme
- 0.5 mL Anza 10X T4 PNK Buffer