Invitrogen™ Anza™ DNA Blunt End Kit

Invitrogen™ Anza™ DNA Blunt End Kit

The Invitrogen™ Anza™ DNA Blunt End Kit is used to convert DNA with cohesive ends to DNA with blunt ends for use in blunt-end ligation reactions. The included Anza™ DNA Blunting Enzyme Mix contains T4 DNA polymerase and Klenow Fragment, while the Anza™ 10X Blunting Buffer contains dNTPs to facilitate the synthesis of blunt ends. Use this kit when the desired vector does not contain a recognition site that would produce blunt ends.

Features

• Fast 15-minute, room-temperature protocol
• DNA digested with Anza™ restriction enzymes can be used directly in the Anza Blunt End protocol following heat inactivation

Contents

• 100 µL Anza Blunting Enzyme Mix
• 200 µL Anza 10X Blunting Buffer