Invitrogen™ BacLight™ Bacterial Membrane Potential Kit

Invitrogen™ BacLight™ Bacterial Membrane Potential Kit

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

Contents

• DiOC2(3): 1.2 mL of 3 mM solution in DMSO.
• CCCP: 300 μL of 500 μM solution in DMSO.
• PBS: 200 mL.