Invitrogen™ CellROX™ Deep Red Flow Cytometry Assay Kit, 100 Assays

Invitrogen™ CellROX™ Deep Red Flow Cytometry Assay Kit, 100 Assays

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.

Features

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS.
• Multicolor compatibility, minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents.
• Simple protocol, cells can be stained in complete media or other appropriate buffer, no need for serum free media.

Contents

• 1 vial CellROX™ Deep Red Reagent (25 µL).
• 1 vial SYTOX™ Blue Dead Cell Stain (100 µL).
• 2 vials N-acetylcysteine (10 mg per vial).
• 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water).
• 1 vial DMSO (200 µL).