Invitrogen™ BaculoDirect™ C-Term Expression Kit

Invitrogen™ BaculoDirect™ C-Term Expression Kit

The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway™ Technology with baculovirus expression makes the BaculoDirect System the fastest and easiest method for production of recombinant baculovirus.

Features

• Strong polyhedrin promoter for high-level expression
• R-sites for efficient recombination with any attL-flanked Gateway entry vector
• TK gene for negative selection using ganciclovir
• C-terminal V5-His tag (BaculoDirect™ C-Term Expression Kit) or N-terminal V5-His tag (BaculoDirect™ N-Term Expression Kit) for detection with anti-V5 antibody and purification with nickel-chelating resin
• TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect N-Term Expression Kit)
• LacZ gene, to ensure a pure baculovirus stock is generated, which is replaced by your gene of interest after LR recombinant reaction

Contents

• The BaculoDirect expression kits include five 0.3 µg vials of BaculoDirect Linear DNA, 125 µL of Cellfectin transfection reagent, frozen Sf9 cells, Gateway LR Clonase enzyme mix, GIBCO Unsupplemented Grace's Insect Medium, and ganciclovir