Invitrogen™ Jump-In™TI™ Platform Kit

Invitrogen™ Jump-In™TI™ Platform Kit

The Jump-In™TI™ Platform Kit enables generation of a Jump-In™TI™ cell line. A Jump-In™TI™ cell line is created by co-transfecting one of three vectors containing the R4 attP target site with the transiently expressed vector for the PhiC31 integrase into your mammalian cell line.

Generating a Jump-In™TI™ cell line is the first step in creating a stable, targeted, and irreversible mammalian cell line expressing your gene of interest. For targeted and irreversible integration of your gene of interest into your Jump-In™TI™ cell line, use the Jump-In™TI™ Gateway® Vector Kit.

This kit is also a component of the Jump-In™TI™ Gateway® System.

Contents

The Jump-In™TI™ Platform Kit is composed of three vectors:

• pJTI™⁄Bsd Vector (500 ng⁄µl; total: 20 µl),
• pJTI™⁄Neo Vector (500 ng⁄µl; total: 20 µl),
• pJTI™⁄Zeo Vector (500 ng⁄µl; total 20 µl),
• pJTI™ PhiC31 Int Vector (0.5 µg⁄µl; total 20 µl)