Invitrogen beta-3 Tubulin Monoclonal Antibody (2G10)

Invitrogen beta-3 Tubulin Monoclonal Antibody (2G10)

MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.

MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.

Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.