Invitrogen beta Actin Monoclonal Antibody (BA3R), DyLight™ 550, 50 µL
On demandInvitrogen beta Actin Monoclonal Antibody (BA3R), DyLight™ 550, 50 µL
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
MA5-15739-D550 has been successfully used for Immunofluorescence. DyLight 550 has an excitation/emission of 562/576nm.
For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.
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