Invitrogen beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 488, 50 µL
On demandInvitrogen beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight™ 488, 50 µL
Beta actin is one of six actin
isoforms which have been identified. Beta actin is a non-muscle
cytoskeletal protein in all human cell types and is involved in cell
motility, structure, and integrity. There are six different actin
isoforms in human. The beta isoform of actin, along with gamma actin,
coexist in most cell types as components of the cyto-skeleton. Beta
actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic
cells. Polymerization of globular actin (G-actin) leads to a structural
filament (F-actin) in the form of two stranded helix. Actins are highly
conserved proteins that are involved in cell motility, structure and
integrity. Because beta actin is ubiquitously expressed in all
eukaryotic cells, it is frequently used as a loading control for assays
involving protein detection, such as Western blotting. Antibodies to
beta-actin provide a specific and useful tool in studying the
intracellular distribution of beta-actin and the static and dynamic
aspects of the cytoskeleton.
MA5-15739-D488 has been successfully used in immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.
For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.
There are no specifications
There are no report
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