Invitrogen BSSP 4 (PRSS22) Mouse ELISA Kit, 10 x 96 Tests

Invitrogen BSSP 4 (PRSS22) Mouse ELISA Kit, 10 x 96 Tests

The Mouse BSSP 4 (PRSS22) ELISA quantitates Ms BSSP 4 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BSSP 4.

Principle of the method

The Mouse BSSP 4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: <12%
• Intraassay CV: <10%
• Homogenous (no wash): No
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Mouse BSSP 4 Antibody Coated wells, 96-well plate, 1 plate
• Mouse BSSP 4 Biotin Conjugate, 2 vials
• Mouse BSSP 4 Standard, recombinant mouse BSSP 4, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 15 mL
• Streptavidin-HRP (300X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2