Invitrogen IL-1 beta-Cell Lysates Rat ELISA Kit, 5 x 96 Tests
On demandInvitrogen IL-1 beta-Cell Lysates Rat ELISA Kit, 5 x 96 Tests
The Rat Interleukin-1beta-Cell Lysates (IL-1β) ELISA quantitates rat IL-1β in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant rat IL-1β.
Principle of the method
The rat IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
Contents
- Pre-coated 96 well plate
- Standard
- Assay Diluent concentrate
- Biotinylated Detection Antibody
- SAV-HRP
- Cell Lysis Buffer
- Wash Buffer
- Chromogen
- Stop Solution
- Adhesive Plate Covers
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