Invitrogen c-Met (Soluble) Human ELISA Kit, 96 Tests

Invitrogen c-Met (Soluble) Human ELISA Kit, 96 Tests

The Human c-Met (Soluble) ELISA quantitates Hu c-Met in human serum, plasma and cell culture supernatant. The assay will exclusively recognize both natural and recombinant Hu c-Met.

Principle of the method

The Human c-Met solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 8.1%
• Interassay CV: 6.1%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Hu c‑Met (soluble) Standard, lyophilized; contains 0.1% sodium azide. 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide and red dye[1], 60 mL
• Antibody Coated Plate, 96-well plate, 1 plate
• Hu c‑Met (soluble) Biotin Conjugate; contains 0.1% sodium azide and blue dye[1], 11 mL
• Streptavidin‑HRP (100X), 0.15 mL
• HRP Diluent; contains 3.3 mM thymol and yellow dye[1], 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 4