Invitrogen c-Myc (Total) Human ELISA Kit, 96 Tests
On demandInvitrogen c-Myc (Total) Human ELISA Kit, 96 Tests
The Human c-Myc Total (Hu c-Myc) ELISA quantitates Hu c-Myc in human cell lysates. The assay will exclusively recognize both natural and recombinant Hu c-Myc, independed of phosphorylation state.
Principle of the method
The Human c-Myc solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: 3.7%
- Interassay CV: 4.9%
- Homogenous (no wash): No
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu c‑Myc (Total) Standard; lyophilized. Refer to vial label for reconstitution volume, 1 vial
- Standard Diluent Buffer; contains 0.1% sodium azide and red dye[1], 25 mL
- Hu c‑Myc (Total) Antibody Coated Plate; 96-well plate, 1 plate
- Rabbit anti‑Hu c‑Myc Detection Antibody; contains 0.1% sodium azide and blue dye[1], 6 mL
- Anti-Rabbit IgG HRP (100X); contains 3.3 mM thymol, 0.125 mL
- HRP Diluent; contains 3.3 mM thymol and yellow dye[1], 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 3
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