Invitrogen C-Peptide Human ELISA Kit, 96 Tests

Invitrogen C-Peptide Human ELISA Kit, 96 Tests

The Human c-Peptide ELISA quantitates Hu c-Peptide in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu c-Peptide.

Principle of the method

The Human c-Peptide solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 10.5%
• Interassay CV: 5.5%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard (2 vials)
• Standard Dilution Buffer
• Biotinylated Detection Antibody
• Streptavidin-HRP
• HRP Diluent
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers