Invitrogen CD40 Monoclonal Antibody (1C10), PerCP-eFluor 710, 100 µg, eBioscience™
On demand
Order number :
46-0401-82
Invitrogen CD40 Monoclonal Antibody (1C10), PerCP-eFluor 710, 100 µg, eBioscience™
The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Features
- PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
- Recommended Isotype Control: Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor 710, eBioscience™.
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