Invitrogen CD40L (Soluble) Human Instant ELISA Kit, 128 Tests

Invitrogen CD40L (Soluble) Human Instant ELISA Kit, 128 Tests 

The Human CD40 Ligand (Hu CD40L)) ELISA quantitates Hu CD40L in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD40L.

Principle of the method

The Human CD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 14.2%
• Intraassay CV: 6.8%
• Storage: -20°C

Contents

• Pre-coated 96 well Microplate
• Detection Antibody
• Anti-IgG-HRP
• Sample Diluent, lyophilized
• Standard (8-well strips x 4)
• Wash Buffer Concentrate
• Substrate Solution
• Sample Diluent
• Stop Solution
• Lysis Buffer 10x
• Adhesive Plate Covers