Invitrogen CD45RO Monoclonal Antibody (UCHL1), PerCP-eFluor™ 710, 100 Tests, eBioscience™

Invitrogen CD45RO Monoclonal Antibody (UCHL1), PerCP-eFluor™ 710, 100 Tests, eBioscience™

The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Filtration: 0.2 µm post-manufacturing filtered.