Invitrogen CD83 Monoclonal Antibody (HB15e), APC-eFluor™ 780, 25 Tests, eBioscience™
On demandInvitrogen CD83 Monoclonal Antibody (HB15e), APC-eFluor™ 780, 25 Tests, eBioscience™
The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells.
APC-eFluor 780 emits at 780 nm and is excited with the Red laser
(633-647 nm). Please make sure that your instrument is capable of
detecting this fluorochrome.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100
µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse
Solution for up to 3 days in the dark at 4°C with
minimal impact on brightness and FRET efficiency/compensation. Some
generalizations regarding fluorophore performance after fixation can be
made, but clone specific performance should be determined empirically.
There are no specifications
There are no report
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