Invitrogen™ CellEvent™ Senescence Green Flow Cytometry Assay Kit, 200 Assays
On demandInvitrogen™ CellEvent™ Senescence Green Flow Cytometry Assay Kit, 200 Assays
The CellEvent Senescence Green Flow Cytometry Assay Kit enables flow cytometric detection of cellular senescence via β-galactosidase hydrolysis. The kit contains an optimized buffer and a fluorescein-based reagent that contains two galactoside moieties, making it a specific target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzyme-cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.
Features
- Fluorescent detection of β-galactosidase hydrolysis by flow cytometry, When normal cells reach the end of their limited replicative lifespan,
commonly referred to as the Hayflick limit, they enter into a senescent
phase where they remain metabolically active without undergoing cell
death processes. These senescent cells adopt a specific phenotypic state
that includes the appearance of multi-nucleated cells, increased
vacuolization, expression of pH-dependent β-galactosidase, and
morphological changes where cells become enlarged and extended. Through a
variety of mechanisms, they may play a role in tumor suppression, tumor
progression, aging, and tissue repair.
β-galactosidase resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The activity is optimal under lysosomal pH 4, but conventional assays measure at pH 6. It has been shown that normalized β-galactosidase activity is twice as high in senescent cells as in presenescent cells regardless of the pH value used for testing. - Fixed cell assay.
- Multi-color compatibility.
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