Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, Alexa Fluor™ 488, Propidium Iodide, 50 Assays

Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, Alexa Fluor™ 488, Propidium Iodide, 50 Assays

The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI) is used in flow cytometry to measure early apoptosis by detecting phosphatidyl serine (PS) expression and membrane permeability. When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.

Features

• Superior brightness, Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).
• High binding efficiency, Annexin V conjugates are made from a highly purified cys-annexin, resulting in higher binding efficiency and extremely accurate characterization of the apoptotic process.
• Multi-parametric, Many publications require a minimum of two different ways to identify apoptotic cells. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane, while membrane integrity is determined via propidium iodide.

Contents

• 1 vial of annexin V, Alexa Fluor 488 conjugate (250 µL).
• 1 vial of propidium iodide (PI, 100 µL).
• 1 bottle of annexin binding buffer (5X solution, 15 mL).