Invitrogen™ Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy

Invitrogen™ Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy

The Image-iT™ LIVE Green Reactive Oxygen Species (ROS) Detection Kit provides the key reagents necessary for the detection of ROS in live cells. The assay is based on 5-(and-6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA), a reliable fluorogenic marker for ROS in live cells. In addition to carboxy-H2DCFDA, the kit provides the common inducer of ROS production tert-butyl hydroperoxide (TBHP), as a positive control and the blue-fluorescent, cell-permeant nucleic acid stain Hoechst 33342

Contents

• Component A: 5-(and-6)-carboxy-2′,7′-dichlorodihydro- fluorescein diacetate (carboxy-H2DCFDA), 5 vials each containing 275 μg
•  Component B: Hoechst 33342, 400 μL of a 1 mM solution
• Component C: tert-butyl hydroperoxide (TBHP) solution, 50 μL of a 7.78 M solution
• Component D: Dimethylsulfoxide (DMSO), 500 μL