Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit, 20 Preps

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit, 20 Preps

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

This new addition to the family includes a Power SYBR™ Green 1-Step RT-PCR master mix. The 1-step format of the RT-PCR step streamlines the Cells-to- CT™ 'sample to answer' workflow more than ever without compromising sensitivity and specificity and at an extraordinary value.

For use with equipment: 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system

Features

• Complete—optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR™ Green 1-Step RT-PCR Master Mix
• Faster—7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
• Simple—12 steps total (sample prep and real-time RT-PCR combined)
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Affordable—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample prepared; study multiple targets per sample at an affordable price

Contents

• Lysis Solution, 2.2 mL, store at 2–8°C
• Dnase I, 22 µL, store at -5 to -30°C
• Stop Solution, 200 µL, store at -5 to -30°C
• Power SYBR™ Green 1-Step qPCR Mix, 500 µL, store at -5 to -30°C
• Power SYBR™ Green 1-Step RT Mix, 10 µL, store at -5 to -30°C