Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit, 400 Preps
On demandInvitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit, 400 Preps
Like
other members of the Cells-to-CT™ product family, the Cells-to-CT™
1-Step TaqMan™ Kit makes it possible to perform expression analysis
directly from cultured cells without RNA purification.
This
new addition to the family includes a TaqMan™ 1-Step RT-PCR master mix.
The single-tube format of the RT-PCR step streamlines the “sample to
answer” workflow more than ever. Combining the simple Cells-to- CT™
lysis technology with the fast cycling capability of the 1-step RT-PCR
master mix allows quicker time to results with limited sample handling.
For use with equipments: 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Features
- Complete—optimized workflow includes cell lysis reagents with gDNA removal and TaqMan™ 1-Step RT-PCR Master Mix
- Faster than ever—7-minute sample prep, including DNase treatment, combined with a 28-minute real-time RT-PCR step allows for ∼35 minute sample-to-answer workflow
- Simple—10 steps total (sample prep and real-time RT-PCR combined)
- Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
- Convenient—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample you prepare; this allows you to study multiple targets or perform replicate RT-PCRs
Contents
- Lysis Solution, 22 mL, store at 2–8°C
- Dnase I, 220 µL, store at -5 to -30°C
- Stop Solution, 2 x 1 mL, store at -5 to -30°C
- TaqMan™ 1-Step qRT-PCR Mix, 10 mL, store at -5 to -30°C
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