Invitrogen™ ChargeSwitch™ gDNA Mini Bacteria Kit Green Features
On demandInvitrogen™ ChargeSwitch™ gDNA Mini Bacteria Kit
The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic bead-based technology to provide rapid and efficient purification of up to 12 µg of genomic DNA from up to 0.5 ml of Gram-positive or Gram-negative bacterial culture (Figure 1) or an individual colony in less than 15 minutes after lysate preparation.
The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds.
The ChargeSwitch® gDNA Mini Bacteria Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack (CS15000), which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.
Features
- Optimized for higher-purity DNA extraction
- Improved performance with accompanying magnetic rack
- Cleaner purified plasmid for optimal downstream performance
- Purification Target: Genomic DNA
- Sample Type: Bacteria
- Green Features: Fewer resources used and less waste, Less hazardous
Contents
- ChargeSwitch® Magnetic Beads: 2 mL
- Proteinase K: 0.5 mL
- RNase A: 0.4 mL
- ChargeSwitch® Resuspension Buffer (R4): 10 mL
- ChargeSwitch® Lysis Buffer (L14): 25 mL
- ChargeSwitch® Binding Buffer (B8): 20 mL
- ChargeSwitch® Wash Buffer (W12): 100 mL
- ChargeSwitch® Elution Buffer: 10 mL
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