Invitrogen CREB (Total) Human ELISA Kit, 96 Tests
On demandInvitrogen CREB (Total) Human ELISA Kit, 96 Tests
The Human CREB (Total) ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of CREB (Total) in fresh or frozen human cell lysates. Cross-reactivity has been observed in mouse cells. The assay recognizes both natural and recombinant CREB (Total), independent of its phosphorylation state.
Principle of the method
A
monoclonal capture antibody specific for CREB (Total) has been coated
onto the wells of the 96-well plate. During the first incubation,
standards of known content and unknown samples are pipetted into the
wells and the antigen binds to the immobilized (capture) antibody. After
washing, a rabbit antibody specific for the target protein is added to
the wells and serves as a detection antibody by binding to the
immobilized protein captured during the first incubation. After washing,
a horseradish peroxidase labeled anti-rabbit IgG is added. This binds
to the detection antibody to complete the four member sandwich. After a
third incubation and washing to remove all the unbound enzyme, a
substrate solution (TMB) is added, which is acted upon by the bound
enzyme to produce color. The intensity of this colored product is
directly proportional to the concentration of target protein present in
the original specimen and the optical density can be read on a standard
microplate reader.
Features
- Interassay CV: 8.5%
- Intraassay CV: 3.9%
- Homogenous (no wash): No
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- CREB (Total) Standard, lyophilized; contains 0.1% sodium azide. 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
- Antibody Coated Plate, 96-well plate, 1 plate
- CREB (Total) Detection Antibody; contains 0.1% sodium azide, 11 mL
- Anti-Rabbit IgG HRP (100X); contains 3.3 mM thymol, 0.125 mL
- HRP Diluent; contains 3.3 mM thymol, 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Plate Covers, adhesive strips, 3
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