Invitrogen CREB (Phospho) [pS133] Human ELISA Kit, 96 Tests

Invitrogen CREB (Phospho) [pS133] Human ELISA Kit, 96 Tests

The Human CREB (Phospho) [pS133] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of CREB (Phospho) [pS133] in fresh or frozen human cell lysates. Cross-reactivity has been observed in mouse cells. The assay recognizes both natural and recombinant CREB (Phospho) [pS133].

Principle of the method
A monoclonal capture antibody specific for human CREB (Phospho) [pS133] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.

Features

• Interassay CV: 5.8%
• Intraassay CV: 4%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• CREB [pS133] Standard, lyophilized; contains 0.1% sodium azide. 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Wells, 96-well plate, 1 plate
• CREB [pS133] Detection Antibody; contains 0.1% sodium azide, 11 mL
• Anti-Rabbit IgG HRP (100X), 0.125 mL
• HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3