Invitrogen CRP Rat ELISA Kit, 5 x 96 Tests, 100 µL

Invitrogen CRP Rat ELISA Kit, 5 x 96 Tests, Sample Volume 100 µL 

The Rat C-Reactive Protein (Rt CRP) ELISA quantitates Rt CRP in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt CRP.

Principle of the method

The Rat CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%    
• Intraassay CV: <10%    
• Shipping condition: Wet or Dry Ice    
• Storage: 2-8°C

Contents    

• Rat C-reactive protein Antibody Coated wells, 96-well plate, 1 plate
• Rat C-reactive protein Biotin Conjugate, 2 vials
• Rat C-reactive protein Standard, recombinant rat c-reactive protein, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 2 x 15 mL
• Streptavidin-HRP (500X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2