Invitrogen™ CyQUANT™ Direct Red Cell Proliferation Assay, 10 microplates

Invitrogen™ CyQUANT™ Direct Red Cell Proliferation Assay, 10 microplates

The CyQUANT Direct Red Cell Proliferation Assay is a simple no-wash, add-and-read fluorescent microplate assay that can be used to assess cell growth, viability, or compound toxicity. The assay consists of a cell-permeant DNA-binding red fluorescent probe and a cell-impermeant background suppressor. The probe and suppressor are mixed and added to cells; the probe stains all cells, while the suppressor only enters dead cells or cells with compromised cell membranes, suppressing probe fluorescence in those locations. The result is that only healthy cells are detected

Features

• Convenient—no washes, cell lysis, or temperature equilibration; simply add and read to detect changes in adherent and suspension cell proliferation
• Accurate―based on amount of cellular DNA, which is highly regulated and independent of the metabolic state of the cells
• Robust―optimized red fluorescent probe and background suppressor result in a highly sensitive assay with a large dynamic range
• Stable―red fluorescent signal stability for seven hours, ideal for large screening assays
• Multiplex enabled―can be combined with spectrally distinct fluorescent or luminescent readouts
• For Use With (Equipment): HTS Reader, Microplate Reader