Invitrogen™ CyQUANT™ LDH Cytotoxicity Assay, 1000 assays

Invitrogen™ CyQUANT™ LDH Cytotoxicity Assay, 1000 assays

The CyQUANT LDH Cytotoxicity Assay is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity. Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage to the plasma membrane. The CyQUANT LDH Cytotoxicity Assay provides the reagents to accurately and quantitatively measure this extracellular LDH

Features

• Convenient—add-mix-read assay format for adherent and suspension cells, including 3D cell models
• Accurate—provides a quantitative measurement of LDH release
• Flexible—ideal for high-throughput screening, monitor cytotoxicity from the same sample over time
• Robust—uses stable LDH enzyme activity as a cytotoxic marker
• For Use With (Equipment): Microplate Reader

Contents

• Substrate Mixture, 5 vials lyophilizate
• Assay Buffer, 5 x 600 μL
• Lysis Buffer, 12 mL
• Stop Solution, 60 mL
• LDH Positive Control, 30 μL