Invitrogen™ CyQUANT™ XTT Cell Viability Assay

Invitrogen™ CyQUANT™ XTT Cell Viability Assay

The CyQUANT XTT Cell Viability Assay is a complete, optimized assay that generates a consistent colorimetric detection of viable mammalian cells. The assay kit consists of two reagents, XTT Reagent and Electron Coupling Reagent. XTT Reagent is used to assess cell viability as a function of cellular redox potential, and the electron coupling reagent improves the dynamic range of the assay. The assay is sufficient for ten 96-well plates and consists of 10 bottles of XTT Reagent and 10 tubes of Electron Coupling Reagent. One bottle of each (sufficient for one 96 well plate) are thawed, mixed together, added to cells, and incubated. The viability signal is detected using an absorbance-based microplate reader. In actively respiring viable mammalian cells, the water-soluble XTT reagent is reduced and converted to an orange-colored formazan product

Features

• Highly reproducible, absorbance-based viability assay
• Improved dynamic range and sensitivity compared to other colorimetric viability assays
• Simple mix-and-read format, configured for one 96-well plate at a time
• Non-toxic, continuous assay—multiple time points can be collected, no cell lysis
• For Use With (Equipment): Microplate Reader