Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

ReadyProbes™ Cell Viability Imaging Kit (Blue/Green) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue™ Live reagent (Hoechst 33342) and NucGreen™ Dead reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen™ Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set. This kit is appropriate for fluorescence microscopy, fluorescence microplate readers, and flow cytometry. NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)
NucGreen™ Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523 nm)

For Use With (Equipment): Fluorescence Microscope