Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green
On demandInvitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green
ReadyProbes™ Cell Viability Imaging Kit (Blue/Green) is a ready-to-use
assay that can quickly and easily determine the viability of cells. Just
add 2 drops each of room temperature, stable NucBlue™ Live reagent
(Hoechst 33342) and NucGreen™ Dead reagent to 1 mL of cell growth media,
then determine viability by counting total vs dead cells. NucBlue™ Live
reagent stains the nuclei of all the cells and can be detected with a
standard DAPI filter. NucGreen™ Dead reagent stains only the nuclei of
cells with compromised plasma membrane integrity and is detected using a
standard FITC/GFP (green) filter set. This kit is appropriate for
fluorescence microscopy, fluorescence microplate readers, and flow
cytometry. NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)
NucGreen™ Dead reagent:
stains only the nuclei of dead cells with compromised plasma membranes;
detected with standard FITC/GFP (green) filter set (excitation/emission
maxima: 504/523 nm)
For Use With (Equipment): Fluorescence Microscope
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