Invitrogen™ CytoVista™ Antibody Penetration Buffer

CytoVista™ Antibody Penetration Buffer

CytoVista Antibody Penetration Buffer helps primary and secondary antibodies penetrate the cells of tissue or 3D cell culture when performing immunohistochemistry (IHC) or immuno-labeling. This buffer consists of PBS with 0.2% Triton X-100, 0.3 M glycine, and 20% DMSO. It helps enable sharp and bright images at any depth of tissue or 3D cell culture when used with other CytoVista products.

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

Features

• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins