Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, SYTOX Green, R-PE, 50 Assays

Invitrogen™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry, FITC, Propidium Iodide, 50 Assays

The Dead Cell Apoptosis Kit with Annexin V PE and SYTOX Green detects PS externalization in apoptotic cells during flow cytometry using recombinant annexin V conjugated to the orange fluorescent phycobiliprotein R-PE, while dead cells are detected with SYTOX Green nucleic acid stain. After treatment with both probes, apoptotic cells show orange fluorescence, dead cells show green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the 530/30 nm and 585/42 nm bandpass filters with a 488 nm laser flow cytometer.

Features

• Superior brightness.
• High binding efficiency, Annexin V conjugates are made from a highly purified cys-annexin, resulting in higher binding efficiency and extremely accurate characterization of the apoptotic process.
• Multi-parametric, Many publications require a minimum of two different ways to identify apoptotic cells. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane, while membrane integrity is determined via propidium iodide.

Contents

• 1 vial of annexin V, R-PE conjugate (250 µL).
• 1 vial of SYTOX green stain (100 µL).
• 1 bottle of annexin binding buffer (5X solution, 15 mL).