Invitrogen™ Yeast Vacuole Membrane Marker MDY-64

Invitrogen™ Yeast Vacuole Membrane Marker MDY-64

MDY-64 is a green-fluorescent yeast vacuole membrane stain, The Yeast Vacuole Marker Sampler Kit (Cat. No. Y7531) contains sample quantities of a series of both novel and well established vacuole marker probes that show promise for the study of yeast cell biology. Our recent experiments have demonstrated that several membrane- permeable chloromethyl coumarin derivatives are largely sequestered into yeast vacuoles. This kit includes samples of our CellTracker™ Blue CMAC and the aminopeptidase substrate CMAC-Ala-Pro.1-3 Each of these coumarin-based vacuole markers contains a mildly reactive chloromethyl moiety that reacts with accessible thiols on peptides and proteins to form an aldehyde-fixable conjugate. The conjugate formed with CellTracker™ Blue CMAC is blue fluorescent, whereas the CMAC-Ala-Pro substrates require subsequent protease cleavage to activate their fluorescence. The probes selectively stain the lumen of the yeast vacuole. To complement the blue fluorescent staining of the lumen, we provide a new proprietary green fluorescent yeast vacuole membrane marker MDY-64 for staining the vacuole membrane. This kit also includes the commonly used vacuole marker 5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA) as a standard.

Features

• For Use With (Equipment): Fluorescence Microscope
• SubCellular Localization: Vacuoles